OBJECTIVE: Thalassemia is one of the most common genetic disorders worldwide. Cap +1 mutation which causes ‘silent beta thalassemia’ is present around all ethnic groups of Pakistan. This study was designed to detect the frequency of Cap+1 mutation in Pakistani Population.METHODS: Molecular genetic for Cap+1 beta thalassemic mutation was done by extracting DNA from whole blood by using Genomic DNA Purification Kit (Gentra system USA). Amplification Refractory Mutation System (ARMS) primers were designed for detection of normal and mutant DNA.Basic hematological parameters were performed by using automated analyzer (Sysmex KX-21). Cellulose acetate hemoglobin electrophoresis was done by using semi-automated technique (INTERLAB Roma Microtech Series Electrophoresis system 4.23).RESULTS: The frequency of Cap+1 mutation was observed 5% (10/200) in targeted thalassemic families (having patients with beta-thalassemia intermedia) while its frequency was observed 2% (12/600) in total thalassemic genes in Pakistani population.CONCLUSION: Cap+1 (A-C) is a silent mutation and it has very minimum effect on beta globin synthesis because of which it produces very less clinical severity and certain important laboratory diagnostic tests like basic hematological parameters and Hb A2 levels are also remain in normal range. Therefore individuals with Cap+1 mutation may produce children with beta-thalassemia intermedia if they marry an individual with beta-thalassemia minor. Cap+1 (A-C) mutation is an unsuspected cause of beta thalassemia transmission in Pakistani population. This mutation can identify at molecular level. As this molecular defect is difficult to diagnose in Laboratory with routine laboratory tests because of that it has become a serious hindrance for thalassemia prevention program in Pakistan. (Turk J Hematol 2009; 26: 167-70)addictive behavior
adolescent compulsive gambling
gambling click here to see if you are a compulsive online trader
addictive behavior
adolescent compulsive gambling
http://www.800gambler.org/ click here to see if you are a compulsive online trader
addictive behavior
adolescent compulsive gambling
gambling problem click here to see if you are a compulsive online trader
addictive behavior
adolescent compulsive gambling
compulsive gambling. click here to see if you are a compulsive online trader
addictive behavior
adolescent compulsive gambling
compulsive gambling. click here to see if you are a compulsive online trader
buy abortion pill online
cytotec abortion pill buy online
abortion pill buy abortion pill online
buy abortion pill online
cytotec abortion pill buy online
abortion pill buy abortion pill online
buy abortion pill online
cytotec abortion pill buy online
abortion pills buy abortion pill online
mail order viagra without prescription
buy viagra online a href
viagra reviews viagra for sale without a prescription
mail order viagra without prescription
buy viagra online a href
viagra online viagra for sale without a prescription
mail order viagra without prescription
buy viagra online a href
viagra online viagra for sale without a prescription
mail order viagra without prescription
buy viagra online a href
viagra online viagra for sale without a prescription
kamagra without prescription
photos kamagra
kamagra herbal kamagra alternative
kamagra without prescription
photos kamagra
kamagra herbal kamagra alternative
kamagra without prescription
photos kamagra
kamagra herbal kamagra alternative
amoxicillin uk
mail order amoxil
amoxicillin amoxil prescription buy
amoxicillin uk
mail order amoxil
amoxicillin amoxil prescription buy
natural synthroid
synthroid prescription buy
synthroid where to buy thyroxine online
natural synthroid
synthroid prescription buy
natural synthroid where to buy thyroxine online
natural synthroid
synthroid prescription buy
synthroid where to buy thyroxine online
female herbal laxative
order laxative online
laxative get bisacodyl
female herbal laxative
order laxative online
laxative get bisacodyl
ampicillin prescription buy
buy principen in great britain
ampicillin photos ampicillin
ampicillin prescription buy
buy principen in great britain
ampicillin photos ampicillin
ampicillin prescription buy
buy principen in great britain
get ampicillin photos ampicillin
purchase cipro online
get ciprofloxacin
ciprofloxacin cheap ciprofloxacin
purchase cipro online
get ciprofloxacin
ciprofloxacin cheap ciprofloxacin
purchase cipro online
get ciprofloxacin
order cipro cheap ciprofloxacin
cleocin online shop in uk
get clindamycin avoid prescription
clindamycin cleocin online
cleocin online shop in uk
get clindamycin avoid prescription
clindamycin cleocin online
cleocin online shop in uk
get clindamycin avoid prescription
cheap clindamycin cleocin online
order dexone
female herbal dexone
dexamethasone alternative to dexamethasone
order dexone
female herbal dexone
dexamethasone alternative to dexamethasone
natural duricef
natural Cefadroxil
cefadroxil female free sample Cefadroxil
natural duricef
natural Cefadroxil
cefadroxil female free sample Cefadroxil
natural duricef
natural Cefadroxil
cefadroxil female free sample Cefadroxil
order vantin
natural vantin
cefpodoxime vantin prescription buy
order vantin
natural vantin
cefpodoxime vantin prescription buy
generic ceftin
buy ceftin online a href
cefuroxime buy ceftin online safe
generic ceftin
buy ceftin online a href
cefuroxime buy ceftin online safe
abortion pill buy online
where can i buy abortion pills
abortion pill order abortion pill
abortion pill buy online
where can i buy abortion pills
cytotec abortion order abortion pill
abortion pill buy online
where can i buy abortion pills
cytotec abortion order abortion pill
keflex without prescription
buy keflex online
cephalexin get keflex online
how does chloromycetin work
how to use chloramphenicol
chloramphenicol order chloromycetin online
abortion pills
where can you buy the abortion pill
abortion pill pill for abortion online
abortion pills
where can you buy the abortion pill
abortion pill pill for abortion online
abortion pills
where can you buy the abortion pill
abortion pill pill for abortion online
buy augmentin online
how to use augmentin
augmentin female free sample augmentin
buy augmentin online
how to use augmentin
augmentin female free sample augmentin